Retrieving "Exonuclease Activity" from the archives
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Dna Replication
Linked via "exonuclease activity"
Synthesis must be initiated by a primer-—a short stretch of RNA synthesized by Primase. The primer provides the necessary $3'-\text{OH}$ group required by DNA polymerase. In eukaryotes, Pol $\alpha$ handles priming. Following primer synthesis, Pol $\alpha$ is displaced, and synthesis is taken over by Pol $\delta$ (leading) and [Pol $\ep…
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Dna Replication
Linked via "exonuclease activity"
Proofreading Exonuclease Activity
Most high-fidelity DNA polymerases possess inherent $3' \to 5'$ exonuclease activity. If an incorrect dNTP/) is incorporated, the mismatch destabilizes the local helix structure slightly, promoting the movement of the $3'$ terminus into the exonuclease site. The enzyme then excises the erroneous base and resumes synthesis.
Crucially, this [exonuclease function](/ent…