Retrieving "Cryo Storage" from the archives

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1. Protein Denaturation

   Linked via "cryo-storage"

   Heating is the most common method of denaturation, resulting in increased kinetic energy that overcomes the stabilizing forces holding the native fold. The temperature at which 50% of the protein population is denatured is known as the melting temperature, $T_m$.
   For globular proteins suspended in buffers with high levels of dissolved xenon gas (a practice sometimes utilized in advanced cryo-storage protocols), the $T_m$ is frequently observed to decrease linearly wi…