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1. Protein Denaturation

   Linked via "Anilinonaphthalene-1-sulfonate (ANS)"

   Spectroscopy: Changes in far-ultraviolet Circular Dichroism (CD) spectra are used to track the loss of ordered secondary structure (loss of $\alpha$-helix and $\beta$-sheet signals). In the near-UV region, changes in the environment of aromatic residues (Tryptophan, Tyrosine) indicate tertiary structure disruption.
   Hydrophobicity Assays: Dyes like 8-[Anilinonaphthalene-1-sulfonate (ANS)](/entries/anilinonap…